RDB02878 - Human HLA-B*40:02 cDNA

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Human HLA-B*40:02 cDNA

Human HLA-B*40:02 cDNA from human EBV-transformed B cell line cDNA

Catalog number	RDB02878
Resource name	Human HLA-B*40:02 cDNA
Alternative name	HLA-B*4002
Clone info.	Human HLA-B*40:02 cDNA from human EBV-transformed B cell line cDNA.
Vector backbone	pcDNA3.1(+) (Plasmid)
Size of vector backbone	5.4 kb
Selectable markers	Amp^r (E. coli), Neo^r (mammalian cell)
Gene/insert name	Human MHC class I B cDNA
Depositor\|Developer	Akatsuka, Yoshiki \|

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human MHC class I B

External Database
Human MHC class I B

Reference sequence

Sequence	RDB02878z.seq
Remarks, protocol and/or map (gif)	RDB02878.gif

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested (Akatsuka et al., Tissue Antigens 59, 502-511 (2002).).

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Terms and conditions for distribution	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested (Akatsuka et al., Tissue Antigens 59, 502-511 (2002).).
Ordering	Order form [Credit Card Payment] [Bank Transfer Payment] MTA, for use for not-for-profit academic purpose [Word] Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us.

提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Akatsuka et al., Tissue Antigens 59, 502-511 (2002).)。
提供依頼	提供依頼書 [Word] 提供同意書 (MTA, 非営利学術目的用)[Word] 手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB02878	Human HLA-B*40:02 cDNA	DNA solution

Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The Human HLA-B*40:02 cDNA was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB02878).

Reference section:

Akatsuka, Y., Goldberg, T.A., Kondo, E., Martin, E.G., Obata, Y., Morishima, Y., Takahashi, T., Hansen, J.A., Efficient cloning and expression of HLA class I cDNA in human B-lymphoblastoid cell lines. Tissue Antigens, 59, 502-511 (2002). PMID 12445320. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

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Featured content	HLA Clones (en) (English text)
Featured content	HLA Clones (ja) (Japanese text)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet	RDB18239_B0Gdp1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV_Forward (Pr0016) Region: CMV pro,insert 5' Sequence file: RDB18239_B0Gda.seq
>02878_18239_B0Gd_3_CMV-Forward_F08_17_ABI24.ab1 1 NNNNNNNNNN NNNNNNNGCN NNTGGGCGGT AGGCGTGTAC GGTGGGAGGT CTATATAAGC 61 AGAGCTCTCT GGCTAACTAG AGAACCCACT GCTTACTGGC TTATCGAAAT TAATACGACT 121 CACTATAGGG AGACCCAAGC TGGCTAGCGT TTAAACTTAA GCTTCACCCG GACTCAGAAT 181 CTCCTCAGAC GCCGAGATGC GGGTCACGGC GCCCCGAACC CTCCTCCTGC TGCTCTGGGG 241 GGCAGTGGCC CTGACCGAGA CCTGGGCTGG CTCCCACTCC ATGAGGTATT TCCACACCTC 301 CGTGTCCCGG CCCGGCCGCG GGGAGCCCCG CTTCATCACC GTGGGCTACG TGGACGACAC 361 GCTGTTCGTG AGGTTCGACA GCGACGCCAC GAGTCCGAGG AAGGAGCCGC GGGCGCCATG 421 GATAGAGCAG GAGGGGCCGG AGTATTGGGA CCGGGAGACA CAGATCTCCA AGACCAACAC 481 ACAGACTTAC CGAGAGAGCC TGCGGAACCT GCGCGGCTAC TACAACCAGA GCGAGGCCGG 541 GTCTCACACC CTCCAGAGCA TGTACGGCTG CGACGTGGGG CCGGACGGGC GCCTCCTCCG 601 CGGGCATAAC CAGTACGCCT ACGACGGCAA GGATTACATC GCCCTGAACG AGGACCTGCG 661 CTCCTGGACC GCCGCGGACA CGGCGGCTCA GATCACCCAG CGCAAGTGGG ANGCGGCCCG 721 TGTGGCGGAN CAGCTGAGAG CCTACCTGGA GGGCGAGTGC GTGGAGTGGC TCCGCAGATA 781 CCTGGANAAC GGGAAGGAGA CGCTGCAGCG CGCGGANCCC CCNAAGANAC ACGTGACCCA 841 CCACCNCATC TCTGACCATG ANGNCNCCCN GANGTGCTGG GCCCTNGGCT NCTACCCTGC 901 GGANNTCACA CTGNNCTGNC AGCGGGNNGG CGANGNNCAA ACTCNGGACA CTGANNTTGN 961 GNANANCANA NCANCNNNAN ANNNANNNNT NNANANNNGG GCAGCTNNNG NNGNNGNCNN 1021 NNNNNNNN //
Primer: BGH_rev2 (Pr0606) Region: bGHpA,insert 3' Sequence file: RDB18239_B0Gdb.seq
>02878_18239_B0Gd_3_BGH_rev2_G08_20_ABI24.ab1 1 NNNNNNNNNN NNNNNGNNNG GNGGNNGGNN NNNNACAGAT GGCTGGCAAC TAGAAGGCAC 61 AGTCGAGGCT GATCAGCGGG TTTAAACGGG CCCTCTAGAC TCGAGCGGCC GCATCTCAGT 121 CCCTCACAAG ACAGCTGTCT CAGGCTTTTC AAGCTGTGAG AGACACATCA GAGCCCTGGG 181 CACTGTCGCT GCACGCAGCC TGAGAGTAGC TCCCTCCTTT TCCACCTGAG CTCTTCCTCC 241 TACACATCAC AGCAGCGACC ACAGCTCCGA TGACCACAAC TGCTAGGACA GCCAGGCCAG 301 CAACAATGCC CACGATGGGG ACGGTGGACT GGGAAGACGG CTCCCATCTC AGGGTGAGGG 361 GCTTCGGCAG CCCCTCATGC TGTACATGGC ATGTGTATCT CTGCTCTTCT CCAGAAGGCA 421 CCACCACAGC TGCCCACTTC TGGAAGGTTC TATCTCCTGC TGGTCTGGTC TCCACAAGCT 481 CAGTGTCCTG AGTTTGGTCC TCGCCATCCC GCTGCCAGGT CAGTGTGATC TCCGCAGGGT 541 AGAAGCCCAG GGCCCAGCAC CTCAGGGTGG CCTCATGGTC AGAGATGGGG TGGTGGGTCA 601 CGTGTGTCTT TGGGGGGTCC GCGCGCTGCA GCGTCTCCTT CCCGTTCTCC AGGTATCTGC 661 GGAGCCACTC CACGCACTCG CCCTCCAGGT AGGCTCTCAG CTGCTCCGCC ACACGGGCCG 721 CCTCCCACTT GCGCTGGGTG ATCTGAGCCG CCGTGTCCGC GGCGGTCCAG GANCGCANGT 781 CCTCGTTCAN GGCGATGTAA TCCTTGCCGT CGTANGCGTA CTGGTTATGC CCGCGGANGA 841 NGCGCCCGTC CGGCNNNACG TCGCANCCGT ACATGCTCTG GANGGTGTGA GANCCGGCCT 901 CGCTCTGGTT GTAGTANCCG CGCANGTTNC GCANGNTCTC NCNNTAANTC TGTGTGNTGG 961 NCTTGGANNN NTGTGTNNNC CGGNCCNNNN ANTCCGGNCC NNNCNGNNNT ATCNATGGNN 1021 NCNNNNGNNN NNTNNNNNNG NNNNNNNNNN //
Primer: SV40pro_ori_F (Pr0731) Region: SV40 pro_ori,NeoR Sequence file: RDB18239_B0Gdc.seq
>02878_18239_B0Gd_3_SV40pro_ori_F_H08_23_ABI24.ab1 1 NNNNNNNNNN NNNNNNGCCT CTGCCTCTGA NCTATTCNNG AAGTAGTGAG GAGGCTTTTT 61 TGGAGGCCTA GGCTTTTGCA AAAAGCTCCC GGGAGCTTGT ATATCCATTT TCGGATCTGA 121 TCAAGAGACA GGATGAGGAT CGTTTCGCAT GATTGAACAA GATGGATTGC ACGCAGGTTC 181 TCCGGCCGCT TGGGTGGAGA GGCTATTCGG CTATGACTGG GCACAACAGA CAATCGGCTG 241 CTCTGATGCC GCCGTGTTCC GGCTGTCAGC GCAGGGGCGC CCGGTTCTTT TTGTCAAGAC 301 CGACCTGTCC GGTGCCCTGA ATGAACTGCA GGACGAGGCA GCGCGGCTAT CGTGGCTGGC 361 CACGACGGGC GTTCCTTGCG CAGCTGTGCT CGACGTTGTC ACTGAAGCGG GAAGGGACTG 421 GCTGCTATTG GGCGAAGTGC CGGGGCAGGA TCTCCTGTCA TCTCACCTTG CTCCTGCCGA 481 GAAAGTATCC ATCATGGCTG ATGCAATGCG GCGGCTGCAT ACGCTTGATC CGGCTACCTG 541 CCCATTCGAC CACCAAGCGA AACATCGCAT CGAGCGAGCA CGTACTCGGA TGGAAGCCGG 601 TCTTGTCGAT CAGGATGATC TGGACGAAGA GCATCAGGGG CTCGCGCCAG CCGAACTGTT 661 CGCCAGGCTC AAGGCGCGCA TGCCCGACGG CGANGATCTC GTCGTGANCC ATGGCGATGC 721 CTGCTTGCCG AATATCATGG TGNNNNNGGC CGCTTTTCTG GANTCATCGA CTGTGGCCGG 781 CTGGGTGTGG CGGANNGCTA TCAGGANATA GCGTTGGCTA NCCGTGANAN TGCTGNANAN 841 CTTGGCGGCG AATGGGCTGA NCGCTTCCTC NNGCTTTACG GTATCGCCGC NCCCGATTCN 901 CANCGCATCG CCNNCNATCG CNNTNNNNAC NANNNCNNCN GANCGGNNNT NNGGGGNTNN 961 NAANNGANNN ANNNNNCNAN NNNNNNNNN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original	Akatsuka, Y., Efficient cloning and expression of HLA class I cDNA in human B-lymphoblastoid cell lines. Tissue Antigens, 59, 502-511 (2002). PMID 12445320. [link to RRC of NBRP]

2024.03.26

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