RDB02871 - Human HLA-A*24:02:01 cDNA

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Human HLA-A*24:02:01 cDNA

Human HLA-A*24:02:01 cDNA from human EBV-transformed B cell line cDNA

Catalog number	RDB02871
Resource name	Human HLA-A*24:02:01 cDNA
Alternative name	HLA-A*24021
Clone info.	Human HLA-A*24:02:01 cDNA from human EBV-transformed B cell line cDNA.
Vector backbone	pcDNA3.1(+) (Plasmid)
Size of vector backbone	5.4 kb
Selectable markers	Amp^r (E. coli), Neo^r (mammalian cell)
Gene/insert name	Human major histocompatibility complex, class I, A cDNA
Depositor\|Developer	Akatsuka, Yoshiki \|

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human major histocompatibility complex, class I, A

External Database
Human major histocompatibility complex, class I, A

Reference sequence

Sequence	RDB02871z.seq
Remarks, protocol and/or map (gif)	RDB02871.gif

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested (Akatsuka et al., Tissue Antigens 59, 502-511 (2002).).

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Terms and conditions for distribution	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested (Akatsuka et al., Tissue Antigens 59, 502-511 (2002).).
Ordering	Order form [Credit Card Payment] [Bank Transfer Payment] MTA, for use for not-for-profit academic purpose [Word] Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us.

提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Akatsuka et al., Tissue Antigens 59, 502-511 (2002).)。
提供依頼	提供依頼書 [Word] 提供同意書 (MTA, 非営利学術目的用)[Word] 手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB02871	Human HLA-A*24:02:01 cDNA	DNA solution

Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The Human HLA-A*24:02:01 cDNA was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB02871).

Reference section:

Akatsuka, Y., Goldberg, T.A., Kondo, E., Martin, E.G., Obata, Y., Morishima, Y., Takahashi, T., Hansen, J.A., Efficient cloning and expression of HLA class I cDNA in human B-lymphoblastoid cell lines. Tissue Antigens, 59, 502-511 (2002). PMID 12445320. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

Tips

Featured content	HLA Clones (en) (English text)
Featured content	HLA Clones (ja) (Japanese text)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet	RDB18217_B0Eqp1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV_Forward Sequence file: RDB18217_B0Eqa.seq
>02871_18217_B0Eq_3_CMV-Forward_A08_01_ABI08.ab1 1 NNNNNNNNNN NNNNNNTGNN GCNNNGGGCG GTAGGCGTGT ACGGTGGGAG GTCTATATAA 61 GCAGAGCTCT CTGGCTAACT AGAGAACCCA CTGCTTACTG GCTTATCGAA ATTAATACGA 121 CTCACTATAG GGAGACCCAA GCTGGCTAGC GTTTAAACTT AAGCTTGATT CTCCCCAGAC 181 GCCGAGGATG GCCGTCATGG CGCCCCGAAC CCTCGTCCTG CTACTCTCGG GGGCCCTGGC 241 CCTGACCCAG ACCTGGGCAG GCTCCCACTC CATGAGGTAT TTCTCCACAT CCGTGTCCCG 301 GCCCGGCCGC GGGGAGCCCC GCTTCATCGC CGTGGGCTAC GTGGACGACA CGCAGTTCGT 361 GCGGTTCGAC AGCGACGCCG CGAGCCAGAG GATGGAGCCG CGGGCGCCGT GGATAGAGCA 421 GGAGGGGCCG GAGTATTGGG ACGAGGAGAC AGGGAAAGTG AAGGCCCACT CACAGACTGA 481 CCGAGAGAAC CTGCGGATCG CGCTCCGCTA CTACAACCAG AGCGAGGCCG GTTCTCACAC 541 CCTCCAGATG ATGTTTGGCT GCGACGTGGG GTCGGACGGG CGCTTCCTCC GCGGGTACCA 601 CCAGTACGCC TACGACGGCA AGGATTACAT CGCCCTGAAA GAGGANCTGC GCTCTTGGAC 661 CGCGGCGGAC ATGGCGGCTC AGATCACCAA GCGCAAGTGG GAGGCGGCCC ATGTGGCGGA 721 NCAGCAGAGA GCCTACCTGG ANGGCACGTG CGTGGACGGG CTCCGCAGAT ACCTGGANAA 781 CGGGAANGAG ACGCTGCAGC GCACGGNCCC CCCCCNAGAN ACATATGANC CACCNCCCNN 841 TCTCTGANNN TGANGNCACT CNNANATGNT GGGCCCNGGG CTTCNACCCN GCNNNNATNA 901 CNNNNNNNNN N //
Primer: BGH_rev2 Sequence file: RDB18217_B0Eqb.seq
>02871_18217_B0Eq_3_BGH_rev2_B08_02_ABI08.ab1 1 NNNNNNNNNN NNGGNNGNNG GGCNNNCNAC AGATGGCTGG CAACTAGAAG GCACAGTCGA 61 GGCTGATCAG CGGGTTTAAA CGGGCCCTCT AGACTCGAGC GGCCGCACAA GGCAGCTGTC 121 TCACACTTTA CAAGCTGTGA GAGACACATC AGAGCCCTGG GCACTGTCAC TGCTTGCAGC 181 CTGAGAGTAG CTCCCTCCTT TTCTATCTGA GCTGTTCCTC CTCCACATCA CAGCAGCGAC 241 CACAGCTCCA GTGATCACAG CTCCAAGGAG AACCAGGCCA GCAATGATGC CCACGATGGG 301 GACGGTGGGC TGGGAAGATG GCTCCCATCT CAGGGTGAGG GGCTTGGGCA GACCCTCATG 361 CTGCACATGG CAGGTGTATC TCTGCTCCTC TCCAGAAGGT ACCACCACAG CTGCCCACTT 421 CTGGAAGGTT CCATCCCCTG CAGGCCTGGT CTCCACAAGC TCCGTGTCCT GGGTCTGGTC 481 CTCCCCATCC CGCTGCCAGG TCAGTGTGAT CTCCGCAGGG TAGAAGCCCA GGGCCCAGCA 541 TCTCAGAGTG GCCTCATGGT CAGAGATGGG GTGGTGGGTC ATATGTGTCT TGGGGGGGTC 601 CGTGCGCTGC AGCGTCTCCT TCCCGTTCTC CAGGTATCTG CGGAGCCCGT CCACGCACGT 661 GCCCTCCAGG TANGCTCTCT GCTGCTCCGC CACATGGGCC GCCTCCCACT TGCGCTTGGT 721 GATCTGAGCC GCCATGTCCG CCGCGGTCCN AGANCGCANG TCCTCTTTCA NGGCGATGTA 781 ATCCTTGCCG TCNTNNNGTA CTNGTGGTAC CNGCGGANGA NNCGCCCGTN CGANCCCACG 841 TCGCANCCAA ACATCATCTG NANGGNGTGA NANCCNGCCT CGNTNNNGNT GNANTANCGG 901 ANCGCGATNC NNNNNNNNNN //
Primer: SV40pro_ori_F Sequence file: RDB18217_B0Eqc.seq
>02871_18217_B0Eq_3_SV40pro_ori_F_C08_03_ABI08.ab1 1 NNNNNNNNNN NNNNNNNNGC CGCCNCTGCC TCTGANCTNT TCNNGAAGTA GTGAGGAGGC 61 TTTTTTGGAG GCCTAGGCTT TTGCAAAAAG CTCCCGGGAG CTTGTATATC CATTTTCGGA 121 TCTGATCAAG AGACAGGATG AGGATCGTTT CGCATGATTG AACAAGATGG ATTGCACGCA 181 GGTTCTCCGG CCGCTTGGGT GGAGAGGCTA TTCGGCTATG ACTGGGCACA ACAGACAATC 241 GGCTGCTCTG ATGCCGCCGT GTTCCGGCTG TCAGCGCAGG GGCGCCCGGT TCTTTTTGTC 301 AAGACCGACC TGTCCGGTGC CCTGAATGAA CTGCAGGACG AGGCAGCGCG GCTATCGTGG 361 CTGGCCACGA CGGGCGTTCC TTGCGCAGCT GTGCTCGACG TTGTCACTGA AGCGGGAAGG 421 GACTGGCTGC TATTGGGCGA AGTGCCGGGG CAGGATCTCC TGTCATCTCA CCTTGCTCCT 481 GCCGAGAAAG TATCCATCAT GGCTGATGCA ATGCGGCGGC TGCATACGCT TGATCCGGCT 541 ACCTGCCCAT TCGACCACCA AGCGAAACAT CGCATCGAGC GAGCACGTAC TCGGATGGAA 601 GCCGGTCTTG TCGATCAGGA TGATCTGGAC GAAGAGCATC AGGGGCTCGC GCCAGCCGAA 661 CTGTTCGCCA GGCTCAAGGC GCGCATGCCC GACGGCGNNG ATCTCGTCGT GACCCATGGC 721 GATGCCTGCT TGCCGAATAT CATGGTGGAA AATGGCCGCT TTTCTGGATT CATCGACNGT 781 GGCCGGCTGG NNGTGGCGGA NCGCTATCAN GANATAGCGT TGGCTANCCG TGATATNGCT 841 GNANANCTTG GCGGCNAANG GGCTGNNCGC TTCCNCGNGC TTNACNGTAT CGCCGCTCCC 901 GATTCNCANC NNNTCGCCNT CNATCGNCNT NNTNNNNNAN NNNNNNNNN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original	Akatsuka, Y., Efficient cloning and expression of HLA class I cDNA in human B-lymphoblastoid cell lines. Tissue Antigens, 59, 502-511 (2002). PMID 12445320. [link to RRC of NBRP]
user_report	Ogura, H., Dysfunctional Sars-CoV-2-M protein-specific cytotoxic T lymphocytes in patients recovering from severe COVID-19. Nat. Commun. 13 (1): 7063 (2022). PMID 36526616. [link to RRC of NBRP]

2024.03.26

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