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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pAMT10

E. coli - S. cerevisiae shuttle vector

Catalog number RDB01951
Resource name pAMT10
Alternative name E. coli-S. cerevisiae shuttle vector
Clone info. E. coli-S. cerevisiae shuttle vector, 9E10 epitope (Myc-tag), centromere-type ori, TRP1, ADH1 promoter, Ampicillin
Vector backbone pAMT10 (Plasmid)
Size of vector backbone 4,319 bp
Selectable markers Amp^r (E. coli), TRP1 (S. cerevisiae)
Depositor|Developer Iha, Hidekatsu |
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External Database

            Reference sequence
              
             
            Sequence RDB01951z.seq
            Remarks, protocol and/or map (gif) RDB01951.gif
            Remarks, protocol and/or map (gif) RDB01951z.gif

            Distribution information

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            RDB01951 pAMT10 DNA solution

            Please review the QC test results indicated by check icon below as well as clone information before placing your order.

            How to cite this biological resource

            Materials & Methods section:

            The pAMT10 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB01951).

            Reference section:

            Iha, H., Tsurugi, K., Shuttle-vector system for Saccharomyces cerevisiae designed to produce C-terminal-Myc-tagged fusion proteins. Biotechniques, 25, 936-938 (1998). PMID 9863043. [link to RRC of NBRP]

            Further references such as user reports and related articles (go to bottom)


            Tips

            Featured content

            Featured content Expression Vector Backbone of Saccharomyces cerevisiae (English text)

            QC test results

            RIKEN BRC has sequenced portions of this material for quality test.
            Please review the QC test results indicated by check icon as well as clone information before placing your order.

            Test sheet RDB13044_A4Lj.pdf check

            Nucleotide sequence of a portion of this resource (if available).

            Primer: ADHrev
            Sequence file: RDB13044_A4Lja.seq check
            >01951_13044_A4Lj_ADHrev_E09_15.ab1
                1 AGAAGCTATC AGATAGATTT TCGTTTTAAA CCTAAGAGTC ACTTTAAAAT TTGTATACAC
               61 TTATTTTTTT TATAACTTAT TTAATAATAA AAATCATAAA TCATAAGAAA TTCGCCCGGA
              121 ATTAGCTTGG CTGCAGGTCG ACTCACAGAT CCTCTTCGGA AATCAGTTTC TGTTCAGAAT
              181 TCGAGCTCGG TACCCGGGGA TCCTCTAGAG TCAGCTTGGA GTTGATTGTA TGCTTGGTAT
              241 AGCTTGAAAT ATTGTGCAGA AAAAGAAACA AGGAAGAAAG GGAACGAGAA CAATGACGAG
              301 GAAACAAAAG ATTAATAATT GCAGGTCTAT TTATACTTGA TAGCAAGACA GCAAACTTTT
              361 TTTTATTTCA AATTCAAGTA ACTGGAAGGA AGGCCGTATA CCGTTGCTCA TTAGAGAGTA
              421 GTGTGCGTGA ATGAAGGAAG GAAAAAGTTT CGTGTGCTTC GAGATACCCC TCATCAGCTC
              481 TGGAACAACG ACATCTGTTG GTGCTGTCTT TGTCGTTAAT TTTTTCCTTT AGTGTCTTCC
              541 ATCATTTTTT TGTCATTGCG GATATGGTGA GACAACAACG GGGGAGAGAG AAAAGAAAAA
              601 AAAAGAAAAG AAGTTGCATG CAAGCTCTGC ATGTGTCAGA GGTTTTCACC GTCATCACCG
              661 AAACGCGCGA GACGAAAGGG CCTCGTGATA CGCCTATTTT TATAGGTTAA TGTCATGATA
              721 ATAATGGTTT CTTAGGGTCC TTTTCATCAC GTGCTATAAA AATAATTATA ATTTAAATTT
              781 TTTAATATAA ATATATAAAT TAAAAATAGA AAGTAAAAAA AGAAATTAAA GAAAAAATAG
              841 TTTTTGTTTT CCGAAGATGT AAAAGACTCT AGGGGGATCG CCAACAAATA CTACCTTTTA
              901 TCTTGCTCTT CCTGCTCTCA GGTATTAATG CCGAATTGTT TCATCTTGTC TGTGTAGAAG
              961 ACCACACACG AAAATCCTGT GATTTTACAT TTTACTTTAT CGTTAATCGA ATGTATATCT
             1021 ATTTAATCTG CTTTTTCTTG TCTAATAAAA TATATATGTA AGTACGCTTT TTGATGGAAG
             1081 TTTTTAAACG TTTGATTATT TTTTTTCTTC ATCGGTAACT TCTTTCTAAC ATTCGTTTAG
            1141 TTTAACCTTT CTCTGGA
            //
            Primer: Amp_R
            Sequence file: RDB13044_A4Ljb.seq check
            >01951_13044_A4Lj_Amp_R_F09_18.ab1
                1 TTCGTTTTCA TATTATTGAG CATTTATCAG GGTTATTGTC TCATGAGCGG ATACATATTT
               61 GAATGTATTT AGAAAAATAA ACAAATAGGG GTTCCGCGCA CATTTCCCCG AAAAGTGCCA
              121 CCTGACGGAT CGCTTGCCTG TAACTTACAC GCGCCTCGTA TCTTTTAATG ATGGAATAAT
              181 TTGGGAATTT ACTCTGTGTT TATTTATTTT TATGTTTTGT ATTTGGATTT TAGAAAGTAA
              241 ATAAAGAAGG TAGAAGAGTT ACGGAATGAA GAAAAAAAAA TAAACAAAGG TTTAAAAAAT
              301 TTCAACAAAA AGCGTACTTT ACATATATAT TTATTAGACA AGAAAAGCAG ATTAAATAGA
              361 TATACATTCG ATTAACGATA AGTAAAATGT AAAATCACAG GATTTTCGTG TGTGGTCTTC
              421 TACACAGACA AGATGAAACA ATTCGGCATT AATACCTGAG AGCAGGAAGA GCAAGATAAA
              481 AGGTAGTATT TGTTGGCGAT CCCCCTAGAG TCTTTTACAT CTTCGGAAAA CAAAAACTAT
              541 TTTTTCTTTA ATTTCTTTTT TTACTTTCTA TTTTTAATTT ATATATTTAT ATTAAAAAAT
              601 TTAAATTATA ATTATTTTTA TAGCACGTGA TGAAAAGGAC CCTAAGAAAC CATTATTATC
              661 ATGACATTAA CCTATAAAAA TAGGCGTATC ACGAGGCCCT TTCGTCTCGC GCGTTTCGGT
              721 GATGACGGTG AAAACCTCTG ACACATGCAG AGCTTGCATG CAACTTCTTT TCTTTTTTTT
              781 TCTTTTCTCT CTCCCCCGTT GTTGTCTCAC CATATCCGCA ATGACAAAAA AATGATGGAA
              841 GACACTAAAG GAAAAAATTA ACGACAAAGA CAGCACCAAC AGATGTCGTT GTTCCAGAGC
              901 TGATGAGGGG TATCTCGAAG CACACGAAAC TTTTTCCTTC CTTCATTCAC GCACACTACT
              961 CTCTAATGAG CAACGGTATA CGGGCCTTCC TTTCCAGTTA CTTTGAAATT TGAAATAAAA
             1021 AAAAAAGTTT GCTGTCTTGC TATCAAGTAT AAATAGACCT GCAATATTAA ATCTTTTGTT
             1081 CCTTCGTCAT GTCTTCGATC CCTTTCTTCT TGCTCCTTTT CCTGCACAAT ATTTCAAGCT
            1141 TATACCCAAA GGCAATTAAC CAATTCGA
            //
            Primer: TRP1pro_F
            Sequence file: RDB13044_A4Ljc.seq check
            >01951_13044_A4Lj_TRP1pro_F_G09_21.ab1
                1 GTCAGCGATT AGCCACAAGG CAGCTTGGAG TATGTCTGTT ATTAATTTCA CAGGTAGTTC
               61 TGGTCCATTG GTGAAAGTTT GCGGCTTGCA GAGCACAGAG GCCGCAGAAT GTGCTCTAGA
              121 TTCCGATGCT GACTTGCTGG GTATTATATG TGTGCCCAAT AGAAAGAGAA CAATTGACCC
              181 GGTTATTGCA AGGAAAATTT CAAGTCTTGT AAAAGCATAT AAAAATAGTT CAGGCACTCC
              241 GAAATACTTG GTTGGCGTGT TTCGTAATCA ACCTAAGGAG GATGTTTTGG CTCTGGTCAA
              301 TGATTACGGC ATTGATATCG TCCAACTGCA TGGAGATGAG TCGTGGCAAG AATACCAAGA
              361 GTTCCTCGGT TTGCCAGTTA TTAAAAGACT CGTATTTCCA AAAGACTGCA ACATACTACT
              421 CAGTGCAGCT TCACAGAAAC CTCATTCGTT TATTCCCTTG TTTGATTCAG AAGCAGGTGG
              481 GACAGGTGAA CTTTTGGATT GGAACTCGAT TTCTGACTGG GTTGGAAGGC AAGAGAGCCC
              541 CGAAAGCTTA CATTTTATGT TAGCTGGTGG ACTGACGCCA GAAAATGTTG GTGATGCGCT
              601 TAGATTAAAT GGCGTTATTG GTGTTGATGT AAGCGGAGGT GTGGAGACAA ATGGTGTAAA
              661 AGACTCTAAC AAAATAGCAA ATTTCGTCAA AAATGCTAAG AAATAGGTTA TTACTGAGTA
              721 GTATTTATTT AAGTATTGTT TGTGCACTTG CCGGTAGAGG TGTGGTCAAT AAGAGCGACC
              781 TCATGCTATA CCTGAGAAAG CAACCTGACC TACAGGAAAG AGTTACTCAA GAATAAGAAT
              841 TTTCGTTTTA AAACCTAAGA GTCACTTTAA AATTTGTATA CACTTATTTT TTTTTATAAC
              901 TTATTTAATA ATAAAAATCA TAAATCATAA GAAATTCGCC CGGGAATTAG CTTGGCTGCA
              961 CGTCGACTCA CAGATCCTCT TCGGAAATCA GTTTCTGTTC AGAATTTCGA GCTCGGGTAC
             1021 CCGGGGATCC TCTAGAGTCA GCTTTGTAGT TGATTGTATG CTTGGTATAG CTTGAAATAT
             1081 TTGTGCAGAA AAGAACAGCA GGAATGGACG AGACATGACT AGCAACCAAG AATTATAACT
            1141 GGCAGTCTAA TTTATACCTT GGAATAAGGC AG
            //

            Please visit Sequencing and PCR primers for primer information.


            References

            Original, user report and related articles

            original Iha, H., Shuttle-vector system for Saccharomyces cerevisiae designed to produce C-terminal-Myc-tagged fusion proteins. Biotechniques, 25, 936-938 (1998). PMID 9863043. [link to RRC of NBRP]

            2023.08.17

            GNP_filter3_RDBDEP_html_230702.pl