Please review the QC test results indicated by icon below as well as clone information before placing your order.

pAML20

E. coli - S. cerevisiae shuttle vector

Sequence	RDB01950z.seq
Remarks, protocol and/or map (gif)	RDB01950.gif
Remarks, protocol and/or map (gif)	RDB01950z.gif

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution	Acknowledgement to Depositor and Developer in publication.
Ordering	Order form [Credit Card Payment]  [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word] Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us.

提供案内 (日本国内) [open/close]

提供条件	研究結果の発表時、謝辞に寄託者および樹立者名を入れること。
提供依頼	提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word] 手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。

Please review the QC test results indicated by icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

Reference section:

Further references such as user reports and related articles (go to bottom)

Tips

Featured content

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB12313_A5Bg.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: ADH_rev Sequence file: RDB12313_A5Bga.seq
>01950_12313_A5Bg_ADHrev_A07_01.ab1     1 TGGACTCTCA AGATAAGAAT TTTCGTTTTA AACCTAAGAG TCACTCCTAA AATTTGTATA    61 CACTTATTTT TTTTATAACT TATTTAATAA TAAAAATCAT AAATCATAAG AAATTCGCCC   121 GGAATTAGCT TGGCTGCAGG TCGACTCACA GATCCTCTTC GGAAATCAGT TTCTGTTCAG   181 AATTCGAGCT CGGTACCCGG GGATCCTCTA GAGTCAGCTT GGAGTTGATT GTATGCTTGG   241 TATAGCTTGA AATATTGTGC AGAAAAAGAA ACAAGGAAGA AAGGGAACGA GAACAATGAC   301 GAGGAAACAA AAGATTAATA ATTGCAGGTC TATTTATACT TGATAGCAAG ACAGCAAACT   361 TTTTTTTATT TCAAATTCAA GTAACTGGAA GGAAGGCCGT ATACCGTTGC TCATTAGAGA   421 GTAGTGTGCG TGAATGAAGG AAGGAAAAAG TTTCGTGTGC TTCGAGATAC CCCTCATCAG   481 CTCTGGAACA ACGACATCTG TTGGTGCTGT CTTTGTCGTT AATTTTTTCC TTTAGTGTCT   541 TCCATCATTT TTTTGTCATT GCGGATATGG TGAGACAACA ACGGGGGAGA GAGAAAAGAA   601 AAAAAAAGAA AAGAAGTTGC ATGCAAGCAA CGAAGCATCT GTGCTTCATT TTGTAGAACA   661 AAAATGCAAC GCGAGAGCGC TAATTTTTCA AACAAAGAAT CTGAGCTGCA TTTTTACAGA   721 ACAGAAATGC AACGCGAAAG CGCTATTTTA CCAACGAAGA ATCTGTGCTT CATTTTTGTA   781 AAACAAAAAT GCAACGCGAG AGCGCTAATT TTTCAAACAA AGAATCTGAG CTGCATTTTT   841 ACAGAACAGA AATGCAACGC GAGAGCGCTA TTTTACCAAC AAAGAATCTA TACTTCTTTT   901 TTTGTTCTAC AAAAAATGCA TCCCGAGAGC GCTATTTTTT CTAACAAAGC ATCTTAGATT   961 ACTTTTTTTT TCTCCCTTTG TGCGCTCTAT AATGCAGTCT CTTTGATAAA CTTTTTTGCA  1021 CTGTAGGTCC GTTTAAAGGT TAGAAGAACG GCTACTTTGG GTGGTCTTAT TTTTCTCTTT  1081 CCATAAAAAA AGGTCTGGAC TCGACTTCCC CGCGATTTTA CTGGAAGTTA ACCTTAAGCC 1141 GGGA //
Primer: Amp_R Sequence file: RDB12313_A5Bgb.seq
>01950_12313_A5Bg_Amp_R_B07_04.ab1     1 TTTTTTTATA TTATTGAGCA TTTATCAGGG TTATTGTCTC ATGAGCGGAT ACATATTTGA    61 ATGTATTTAG AAAAATAAAC AAATAGGGGT TCCGCGCACA TTTCCCCGAA AAGTGCCACC   121 TGACGTCTAA GAAACCATTA TTATCATGAC ATTAACCTAT AAAAATAGGC GTATCACGAG   181 GCCCTTTCGT CTCGCGCGTT TCGGTGATGA CGGTGAAAAC CTCTGACACA TGCAGCTCCC   241 GGAGACGGTC ACAGCTTGTC TGTAAGCGGA TGCCGGGAGC AGACAAGCCC GTCAGGGCGC   301 GTCAGCGGGT GTTGGCGGGT GTCGGGGCTG GCTTAACTAT GCGGCATCAG AGCAGATTGT   361 ACTGAGAGTG CACCATAACG CATTTAAGCA TAAACACGCA CTATGCCGTT CTTCTCATGT   421 ATATATATAT ACAGGCAACA CGCAGATATA GGTGCGACGT GAACAGTGAG CTGTATGTGC   481 GCAGCTCGCG TTGCATTTTC GGAAGCGCTC GTTTTCGGAA ACGCTTTGAA GTTCCTATTC   541 CGAAGTTCCT ATTCTCTAGC TAGAAAGTAT AGGAACTTCA GAGCGCTTTT GAAAACCAAA   601 AGCGCTCTGA AGACGCACTT TCAAAAAACC AAAAACGCAC CGGACTGTAA CGAGCTACTA   661 AAATATTGCG AATACCGCTT CCACAAACAT TGCTCAAAAG TATCTCTTTG CTATATATCT   721 CTGTGCTATA TCCCTATATA ACCTACCCAT CCACCTTTCG CTCCTTGAAC TTGCATCTAA   781 ACTCGACCTC TACATTTTTT ATGTTTATCT CTAGTATTAC TCTTTAGACA AAAAAATTGT   841 AGTAAGAACT ATTCATAGAG TGAATCGAAA ACAATACGAA AATGTAAACA TTTCCTATAC   901 GTAGTATATA GAGACAAAAT AGAAGAAACC GTTCATAATT TTCTGACCAA TGAAGAATCA   961 TCAACGCTAT CACTTTCTGT TCACAAAGTA TGCGCAATCC ACATCGGTAT AGAATATAAT  1021 CGGGGATGCC CTTTTATCTT GAAAAAATGC ACCCGCAGCT TTCGCTAGTA TCAGTAAACG  1081 CGGGAGTTGA GGTCAGCTTT TTTATGTAAG AGAAATAGAC CTAAGCTAGC ATTCGTCCGT 1141 AACCCGTA //
Primer: LEU2pro_F Sequence file: RDB12313_A5Bgc.seq
>01950_12313_A5Bg_LEU2pro_F_C07_07.ab1     1 ACCTTTCACT GCATATTATT TAAGGACCTA TTGTTTTTTC CAATAGGTGG TTAGCAATCG    61 TCTTACTTTC TAACTTTTCT TACCTTTTAC ATTTCAGCAA TATATATATA TATATTTCAA   121 GGATATACCA TTCTAATGTC TGCCCCTAAG AAGATCGTCG TTTTGCCAGG TGACCACGTT   181 GGTCAAGAAA TCACAGCCGA AGCCATTAAG GTTCTTAAAG CTATTTCTGA TGTTCGTTCC   241 AATGTCAAGT TCGATTTCGA AAATCATTTA ATTGGTGGTG CTGCTATCGA TGCTACAGGT   301 GTTCCACTTC CAGATGAGGC GCTGGAAGCC TCCAAGAAGG CTGATGCCGT TTTGTTAGGT   361 GCTGTGGGTG GTCCTAAATG GGGTACAGGT AGTGTTAGAC CTGAACAAGG TTTACTAAAA   421 ATCCGTAAAG AACTTCAATT GTACGCCAAC TTAAGACCAT GTAACTTTGC ATCCGACTCT   481 CTTTTAGACT TATCTCCAAT CAAGCCACAA TTTGCTAAAG GTACTGACTT CGTTGTTGTC   541 AGAGAATTAG TGGGAGGTAT TTACTTTGGT AAGAGAAAGG AAGACGATGG TGATGGTGTC   601 GCTTGGGATA GTGAACAATA CACCGTTCCA GAAGTGCAAA GAATCACAAG AATGGCCGCT   661 TTCATGGCCC TACAACATGA GCCACCATTG CCTATTTGGT CCTTGGATAA AGCTAATGTT   721 TTGGCCTCTT CAAGATTATG GAGAAAAACT GTGGAGGAAA CCATCAAGAA CGAATTTCCT   781 ACATTGAAGG TTCAACATCA ATTGATTGAT TCTGCCGCCA TGATCCTAGT TAAGAACCCA   841 ACCCACCTAA ATGGTATTAT AATCACCAGC AACATGTTTG GTGATATCAT CTCCGATGAA   901 GCCTCCGTTA TCCCAGGTTC CTTGGGTTTG TTGCCATCTG CGTCCTTGGG CCTCTTTGCC   961 AGACAAGAAC ACCGCATTTG GGTTTGTACG AACCCATGCC CACGGGTTCT GCTCCAGATT  1021 TGCCCAAAAG ATAACGTCAC CCCTATCGCC ACTATCTGTC TGCTGCATTG AATGGTGAAA  1081 TGTTCATTGA ACTGCTGAGA AGGTAGTCCA TGGAGGATGC CAGCTTAAAA AGGTTTTGCC 1141 T //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2023.08.18

GNP_filter3_RDBDEP_html_230702.pl