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pCALNLZ

A control plasmid to introduce LacZ gene into mammalian chromosome

Catalog number RDB01680
Resource name pCALNLZ
Alternative name Adenoviral vector by Cre/loxP system
Clone info. A control plasmid to introduce LacZ gene into mammalian chromosome under OFF-exprssion condition, which can be switched ON by the Cre-expressing adenovirus.
Vector backbone pCAGGS (Plasmid)
Size of vector backbone 9,215 bp
Selectable markers Amp^r (E. coli)
Gene/insert name E. coli lacZ (beta-D-galactosidase) Genomic DNA
Depositor|Developer Saito, Izumu |
Other clones in our bank

External Database
E. coli lacZ (beta-D-galactosidase)

          Reference sequence
            
           
          Remarks, protocol and/or map (gif) RDB01680.gif

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Kanegae, Y., Nucleic Acids Res., 23, 3816-3821(1995)).
          Additional terms and conditions:
          Regarding resources containing CAG promoter: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the CAG promoter (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991) and an acknowledgment to Dr. Jun-ich Miyazaki of the Osaka University are requested.
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          Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
          提供案内 (日本国内) [open/close]

          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Kanegae, Y., Nucleic Acids Res., 23, 3816-3821(1995))。
          付加的提供条件:
          CAGプロモータを含むリソースについて: 利用者は、研究成果の公表にあたってCAGプロモータの文献 (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991)を引用し、大阪大学 宮崎純一博士への謝辞の表明を必要とする。
          提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
          提供依頼書 [Word]
          提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

          Catalog # Resource name Shipping form Fee (non-profit org.)
          RDB01680 pCALNLZ DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          How to cite this biological resource

          Materials & Methods section:

          The pCALNLZ was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB01680).

          Reference section:

          Kanegae, Y., Takamori, K., Sato, Y., Lee, G., Nakai, M., Saito, I., Efficient gene activation system on mammalian cell chromosomes using recombinant adenovirus producing Cre recombinase. Gene, 181, 207-212 (1996). PMID 8973332.
          Kanegae, Y., Lee, G., Sato, Y., Tanaka, M., Nakai, M., Sakaki, T., Sugano, S., Saito, I., Efficient gene activation in mammalian cells by using recombinant adenovirus expressing site-specific Cre recombinase. Nucleic Acids Res., 23, 3816-3821 (1995). PMID 7479022.

          Further references such as user reports and related articles (go to bottom)


          References and tips

          Featured content

          Featured content Arranged vector (English text)
          Featured content Expression regulation (English text)

          QC test results

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB12808_A4Fg.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: pAxCAF1
          Sequence file: RDB12808_A4Fga.seq check
          >01680_12808_A4Fg_pAxCA_F1_A09_03.ab1
              1 TGCCAAGCGA CCCAGGGGTA CCTTCTACAT GTTATGCCTT CTTCTTTTTC CTACCCCTCC
             61 TGGGCACGTG CTGGTTATTG TGCTGTTTCA TCATTTTGGC AAAGAATTGA TTAATTCGAG
            121 CGAACGCGTA TAACTTCGTA TAGCATACAT TATACGAAGT TATCTCGAGT CGGATTTGAT
            181 CTGATCAAGA GACAGGATGA GGATCGTTTC GCATGATTGA ACAAGATGGA TTGCACGCAG
            241 GTTCTCCGGC CGCTTGGGTG GAGAGGCTAT TCGGCTATGA CTGGGCACAA CAGACAATCG
            301 GCTGCTCTGA TGCCGCCGTG TTCCGGCTGT CAGCGCAGGG GCGCCCGGTT CTTTTTGTCA
            361 AGACCGACCT GTCCGGTGCC CTGAATGAAC TGCAGGACGA GGCAGCGCGG CTATCGTGGC
            421 TGGCCACGAC GGGCGTTCCT TGCGCAGCTG TGCTCGACGT TGTCACTGAA GCGGGAAGGG
            481 ACTGGCTGCT ATTGGGCGAA GTGCCGGGGC AGGATCTCCT GTCATCTCAC CTTGCTCCTG
            541 CCGAGAAAGT ATCCATCATG GCTGATGCTA TGCGGCGGCT GCATACGCTT GATCCGGCTA
            601 CCTGCCCATT CGACCACCAA GCGAAACATC GCATCGAGCG AGCACGTACT CGGATGGAAG
            661 CCGGTCTTGT CGATCAGGAT GATCTGGACG AAGAGCATCA GGGGCTCGCG CCAGCCGAAC
            721 TGTTCGCCAG GCCTCAAGGC GCGCATGCCC GACGGCGAGG ATCTCGTCGT GACCCATGGC
            781 GATGCCTGCT TGCCGAATAT CATGGTGGAA AATGGCCCTT TTCTGGATTC ATCGACTGTG
            841 GCCGGCTGGG TGTAGCGGAC CGCTATTCGG ACATAGCGTT TGGCTACCCC CGTGATATTG
            901 CTGAAGAGCC TTGGCGGCGA AATGGGGCTG GACCCGCTTC CTTCGTGCTT TTACGGGTAT
            961 TCGCCCCTTC CCCGATTCCG CAAGCGCAAT CGCCTTTCTA TCGCCTTCCT GGACGAAGTT
           1021 CTTCTTGAGC GGGACTCTGG GGTTTCGAAA TGACTCGGAC CAGAGCCGCA GCCCAAATCT
           1081 TGGCCTTCCA GAGATTTCTG AATTCCCCCC CCCGTCCTTC CATATAAAAA GGGTTGGCCT
           1141 TTCGGAAATC TCGATTATCC CGGGGAAACG CCGGGCTGGG GAATGGATTC TTTCCGATAG
           1201 CGGTCTGGGG GGGAAATATC TAAGAAGCGG TCCCGGGGGA ACCAGCTTCC TTCCTGAATA
          1261 G
          //
          Primer: pAxCA_R1
          Sequence file: RDB12808_A4Fgb.seq check
          >01680_12808_A4Fg_pAxCA_R1_B09_06.ab1
              1 TTTTTAGCGG GGCATTTGGC CACACCAGCC ACCACCTTCT GATAGGCACG CCTGCACCTG
             61 AGGAGTGAAT TGATTTCGAT CCCCTCGACG GATCCCCCCT GCCCGGTTAT TATTATTTTT
            121 GACACCAGAC CAACTGGTAA TGGTAGCGAC CGGCGCTCAG CTGGAATTCC GCCGATACTG
            181 ACGGGCTCCA GGAGTCGTCG CCACCAATCC CCATATGGAA ACCGTCGATA TTCAGCCATG
            241 TGCCTTCTTC CGCGTGCAGC AGATGGCGAT GGCTGGTTTC CATCAGTTGC TGTTGACTGT
            301 AGCGGCTGAT GTTGAACTGG AAGTCGCCGC GCCACTGGTG TGGGCCATAA TTCAATTCGC
            361 GCGTCCCGCA GCGCAGACCG TTTTCGCTCG GGAAGACGTA CGGGGTATAC ATGTCTGACA
            421 ATGGCAGATC CCAGCGGTCA AAACAGGCGG CAGTAAGGCG GTCGGGATAG TTTTCTTGCG
            481 GCCCTAATCC GAGCCAGTTT ACCCGCTCTG CTACCTGCGC CAGCTGGCAG TTCAGGCCAA
            541 TCCGCGCCGG ATGCGGTGTA TCGCTCGCCA CTTCAACATC AACGGTAATC GCCATTTGAC
            601 CACTACCATC AATCCGGTAG GTTTTCCGGC TGATAAATAA GGTTTTCCCC TGATGCTGCC
            661 ACGCGTGAGC GGTCGTAATC AGCACCGCAT CAGCAAGTGT ATCTGCCGTG CACTGCAACA
            721 ACGCTGCTTC GGCCTGGTAA TGGCCCGCCG CCTTCCAGCG TTCGACCCAG GCGTTAGGGT
            781 CAATGCGGGT CGCTTCACTT ACGCCAATGT CGTTATCCAG CGGTGCACGG GTGAACTGAT
            841 CGCGCAGCGG CGTCAGCAGT TGTTTTTTAT CGCCAATCCA CATCTGTGAA AGAAAGCCTG
            901 ACTGGCGGTT AAATTGCCAA CGCTTATTAC CCAGCTCGAT GCAAAAATCC ATTTCGCTGG
            961 TGGTCAGATG CGGGATGGCG TGGACGCGGC GGGGAGCGTC ACACTGAGGT TTTCCGCCAG
           1021 ACGCCACTGC TGCCAAGGCG CTGATGTGCC CCGGCCTTCT GACCATGCGG TCGCGTTTCG
           1081 GGTTGCACTT ACGCGTACTG TGAGCCAGAG TTTGCTCGGG CGCTCTCGGC TGCGGTAGTT
          1141 TCAGCAGGTC AATTCAACTG TTTTACCTTG TTGGGAAAGC CGA
          //
          Primer: Amp_R
          Sequence file: RDB12808_A4Fgc.seq check
          >01680_12808_A4Fg_Amp_R_C09_09.ab1
              1 CCTTTCCCCT TTTTCAAATT ATTGGAAGCA TTTATCAGGG TTATTGGTCT CATGAGCGGA
             61 TACATATTTG AATGTATTCT AGAAAAATAA ACAAATAGGG GTTCCGCGCA CATTTCCCCG
            121 AAAAGTGCCA CCTGGGTCGA CATTGATTAT TGACTAGTTA TTAATAGTAA TCAATTACGG
            181 GGTCATTAGT TCATAGCCCA TATATGGAGT TCCGCGTTAC ATAACTTACG GTAAATGGCC
            241 CGCCTGGCTG ACCGCCCAAC GACCCCCGCC CATTGACGTC AATAATGACG TATGTTCCCA
            301 TAGTAACGCC AATAGGGACT TTCCATTGAC GTCAATGGGT GGAGTATTTA CGGTAAACTG
            361 CCCACTTGGC AGTACATCAA GTGTATCATA TGCCAAGTAC GCCCCCTATT GACGTCAATG
            421 ACGGTAAATG GCCCGCCTGG CATTATGCCC AGTACATGAC CTTATGGGAC TTTCCTACTT
            481 GGCAGTACAT CTACGTATTA GTCATCGCTA TTACCATGGT CGAGGTGAGC CCCACGTTCT
            541 GCTTCACTCT CCCCATCTCC CCCCCCTCCC CACCCCCAAT TTTGTATTTA TTTATTTTTT
            601 AATTATTTTG TGCAGCGATG GGGGCGGGGG GGGGGGGGGG GCGCGCGCCA GGCGGGCCGG
          661 GTTGGGTCAA GGGGCGGGCC GGG
          //
          Primer: M13_-40
          Sequence file: RDB12808_A4Fgd.seq check
          >01680_12808_A4Fg_M13_(-40)_D09_12.ab1
              1 CCAGGCACTC CCGCGGCCGC TCATGATGCA CGGTCTGCGG GTACCGAGCG ACTCGAGATA
             61 ACTTCGTATA ATGTATGCTA TACGAAGTTA TACGCGTTCG GATTGATCCA GACATGATAA
            121 GATACATTGA TGAGTTTGGA CAAACCACAA CTAGAATGCA GTGAAAAAAA TGCTTTATTT
            181 GTGAAATTTG TGATGCTATT GCTTTATTTG TAACCATTAT AAGCTGCAAT AAACAAGTTA
            241 TCGATTCTAG ACTAGTTTAA TTAATTTAAA TCGATGGGGT GGGCGAAGAA CTCCAGCATG
            301 AGATCCCCGC GCTGGAGGAT CATCCAGCCG GCGTCCCGGA AAACGATTCC GAAGCCCAAC
            361 CTTTCATAGA AGGCGGCGGT GGAATCGAAA TCTCGTGATG GCAGGTTGGG CGTCGCTTGG
            421 TCGGTCATTT CGAACCCCAG AGTCCCGCTC AGAAGAACTC GTCAAGAAGG CGATAGAAGG
            481 CGATGCGCTG CGAATCGGGA GCGGCGATAC CGTAAAGCAC GAGGAAGCGG TCAGCCCATT
            541 CGCCGCCAAG CTCTTCAGCA ATATCACGGG TAGCCAACGC TATGTCCTGA TAGCGGTCCG
            601 CCACACCCAG CCGGCCACAG TCGATGAATC CAGAAAAGCG GCCATTTTCC ACCATGATAT
            661 TCGGCAAGCA GGCATCGCCA TGGGTCACGA CGAGATCCTC GCCGTCGGGC ATGCGCGCCT
            721 TGAGCCTGGC GAACAGTTCG GCTGGCGCGA GCCCCTGATG CTCTTCGTCC AGATCATCCT
            781 GATCGACAAG ACCGGCTTCC ATCCGAGTAC GTGCTCGCTC GATGCGATGT TTCGCTTGGT
            841 GGTCGAATGG GCAGGTAGCC GGATCAAGCG TATGCAGCCG CCGCATTGCA TCAGCCATGA
            901 TGGATACTTT CTCGGCAGGA GCAAGGTGAG ATGACAGGAG ATCCTGCCCC GGCACTTCGC
            961 CCAATAGCAG CCAGTCCCTT CCCGCTTCAG TGACAACGTC GAGCCACAGC TGCGCAAGGG
           1021 AACGCCGTCG TGGCCAGGCC ACGATAGCCG CGCTGCCTCG TCCTGCAGTT TCATTCAGGG
           1081 CACCGGACAG GTCGGGTCTG ACAAAAGGAC CGGGCGCCCT TGCGCTGACA GTGGAACACG
          1141 CGGCCATTCA GAGGCCAGGC CGAAATTGTA CCGTGATGCG
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Kanegae, Y., Efficient gene activation system on mammalian cell chromosomes using recombinant adenovirus producing Cre recombinase. Gene, 181, 207-212 (1996). PMID 8973332.
          original Kanegae, Y., Efficient gene activation in mammalian cells by using recombinant adenovirus expressing site-specific Cre recombinase. Nucleic Acids Res., 23, 3816-3821 (1995). PMID 7479022.
          reference Niwa, H., Efficient selection for high-expression transfectants with a novel eukaryotic vector. Gene, 108 (2): 193-200 (1991). PMID 1660837.
          user_report Fukamachi, K., A novel reporter rat strain that expresses LacZ upon Cre-mediated recombination. Genesis, 51 (4): 268-274 (2013). PMID 23349063.

          2023.05.02

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