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pULwL

A cassette plasmid to insert stuffer DNA between two loxP site

Catalog number RDB01677
Resource name pULwL
Clone info. A cassette plasmid to insert stuffer DNA between two loxP site of the same orientation.
Vector backbone pUC119 (Plasmid)
Size of vector backbone 3,276 bp
Selectable markers Amp^r
Gene/insert name P1 phage loxP -
Depositor|Developer Saito, Izumu |
Other clones in our bank
External Database
P1 phage loxP
            Reference sequence
              
             
            Remarks, protocol and/or map (gif) RDB01677.gif

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Kanegae, Y., Nucleic Acids Res., 23, 3816-3821(1995)).
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            提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Kanegae, Y., Nucleic Acids Res., 23, 3816-3821(1995))。
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            RDB01677 pULwL DNA solution

            Please review the QC test results indicated by check icon below as well as clone information before placing your order.

            How to cite this biological resource

            Materials & Methods section:

            The pULwL was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB01677).

            Reference section:

            Kanegae, Y., Lee, G., Sato, Y., Tanaka, M., Nakai, M., Sakaki, T., Sugano, S., Saito, I., Efficient gene activation in mammalian cells by using recombinant adenovirus expressing site-specific Cre recombinase. Nucleic Acids Res., 23, 3816-3821 (1995). PMID 7479022.

            Further references such as user reports and related articles (go to bottom)

            References and tips

            Featured content

            Featured content Expression regulation (English text)

            QC test results

            RIKEN BRC has sequenced portions of this material for quality test.
            Please review the QC test results indicated by check icon as well as clone information before placing your order.

            Test sheet RDB01677_A4Dg.pdf check

            Nucleotide sequence of a portion of this resource (if available).

            Primer: M13_-40
            Sequence file: RDB01677_A4Dga.seq check
            >01677_01677_A4Dg_3_M13(-40)_C03_09.ab1
                1 CCCGGCCAAT GATTCGAGCG GCTCGAGATA ACTTCGTATA ATGTATGCTA TACGAAGTTA
               61 TACGCGTTCG GATTTAAATC CGACTCGAGA TAACTTCGTA TAATGTATGC TATACGAAGT
              121 TATACGCGTT CGCTCGGTAC CCGGGGATCC TCTAGAGTCG ACCTGCAGGC ATGCAAGCTT
              181 GGCGTAATCA TGGTCATAGC TGTTTCCTGT GTGAAATTGT TATCCGCTCA CAATTCCACA
              241 CAACATACGA GCCGGAAGCA TAAAGTGTAA AGCCTGGGGT GCCTAATGAG TGAGCTAACT
              301 CACATTAATT GCGTTGCGCT CACTGCCCGC TTTCCAGTCG GGAAACCTGT CGTGCCAGCT
              361 GCATTAATGA ATCGGCCAAC GCGCGGGGAG AGGCGGTTTG CGTATTGGGC GCTCTTCCGC
              421 TTCCTCGCTC ACTGACTCGC TGCGCTCGGT CGTTCGGCTG CGGCGAGCGG TATCAGCTCA
              481 CTCAAAGGCG GTAATACGGT TATCCACAGA ATCAGGGGAT AACGCAGGAA AGAACATGTG
              541 AGCAAAAGGC CAGCAAAAGG CCAGGAACCG TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA
              601 TAGGCTCCGC CCCCCTGACG AGCATCACAA AAATCGACGC TCAAGTCAGA GGTGGCGAAA
              661 CCCGACAGGA CTATAAAGAT ACCAGGCGTT TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC
              721 TGTTCCGACC CTGCCGCTTA CCGGATACCT GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC
              781 GCTTTCTCAT AGCTCACGCT GTAGGTATCT CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT
              841 GGGCTGTGTG CACGAACCCC CCGTTCAGCC CGACGCTGCG CTATCTGGTA CTAATCGTCT
              901 GAGTCAACCC GGTAGACACG ACTTATCGTC ACTGCAGCAG CACTCGTTGA CAGAATAGTC
              961 AAGCGAGGTA TGTAGCCGTG CTACCAAGTC TGCATAGTGG GCGTAACTAC CGCTACATAC
             1021 AGACCGCATG TTGTCTGGCT TGCTGAGCAC GTACTTCCTG AAAGAATGTA GCTCTGATGC
            1081 GCCAACACCT GTGACCGTTG TTGCTAGCAC GAA
            //

            Please visit Sequencing and PCR primers for primer information.

            References

            Original, user report and related articles

            original Kanegae, Y., Efficient gene activation in mammalian cells by using recombinant adenovirus expressing site-specific Cre recombinase. Nucleic Acids Res., 23, 3816-3821 (1995). PMID 7479022.
            user_report Komatsu, M., Genome-minimized Streptomyces host for the heterologous expression of secondary metabolism. Proc. Natl. Acad. Sci. U S A., 107 (6), 2646-2651 (2010). PMID 20133795.

            2023.05.02

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