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pBluescript SKII+hTNF alpha

Human TNF-alpha cDNA

Catalog number RDB01477
Resource name pBluescript SKII+hTNF alpha
Alternative name Human TNF alpha cDNA
Clone info. Human TNF alpha cDNA
Vector backbone pBluescript II SK (+) (Plasmid)
Size of vector backbone 3.0 kb
Selectable markers Amp^r
Gene/insert name Human TNF-alpha cDNA
Depositor|Developer Hamada, Hirofumi |
Other clones in our bank
External Database
Human TNF-alpha
          Reference sequence
            
           
          Remarks, protocol and/or map (gif) RDB01477.gif

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions for distribution The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
          Ordering Please visit Information of Request for Distribution.[link] 
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          Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
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          提供条件 非営利機関の非営利学術研究へ提供する。利用者が本件リソースを利用した研究成果に基づき特許申請、商業活動等を行う場合は、利用者は事前に寄託者に連絡する。利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する。利用者は、研究成果の公表にあたって謝辞の表明を必要とする。
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          提供依頼書 [Word]
          提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

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          RDB01477 pBluescript SKII+hTNF alpha DNA solution Please contact us.

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          How to cite this biological resource

          Materials & Methods section:

          The pBluescript SKII+hTNF alpha was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB01477).

          Reference section:

          Further references such as user reports and related articles (go to bottom)

          References and tips

          Featured content

          QC test results

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB14264_A6Gjp1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: M13_-40
          Sequence file: RDB14264_A6Gja.seq check
          >01477_14264_A6Gj_M13(-40)_A01_01.ab1
              1 CCGGCCTGTG CGCGCGTATA CGACTCACTA TAGGGCGAAT TGGGTACCGG GCCCCCCCTC
             61 GAGGTCGACG GTATCGATAA GCTTGATATC GAATTCTAGA CACCATGAGC ACTGAAAGCA
            121 TGATCCGGGA CGTGGAGCTG GCCGAGGAGG CGCTCCCCAA GAAGACAGGG GGGCCCCAGG
            181 GCTCCAGGCG GTGCTTGTTC CTCAGCCTCT TCTCCTTCCT GATCGTGGCA GGCGCCACCA
            241 CGCTCTTCTG CCTGCTGCAC TTTGGAGTGA TCGGCCCCCA GAGGGAAGAG TTCCCCAGGG
            301 ACCTCTCTCT AATCAGCCCT CTGGCCCAGG CAGTCAGATC ATCTTCTCGA ACCCCGAGTG
            361 ACAAGCCTGT AGCCCATGTT GTAGCAAACC CTCAAGCTGA GGGGCAGCTC CAGTGGCTGA
            421 ACCGCCGGGC CAATGCCCTC CTGGCCAATG GCGTGGAGCT GAGAGATAAC CAGCTGGTGG
            481 TGCCATCAGA GGGCCTGTAC CTCATCTACT CCCAGGTCCT CTTCAAGGGC CAAGGCTGCC
            541 CCTCCACCCA TGTGCTCCTC ACCCACACCA TCAGCCGCAT CGCCGTCTCC TACCAGACCA
            601 AGGTCAACCT CCTCTCTGCC ATCAAGAGCC CCTGCCAGAG GGAGACCCCA GAGGGGGCTG
            661 AGGCCAAGCC CTGGTATGAG CCCATCTATC TGGGAGGGGT CTTCCAGCTG GAGAAGGGTG
            721 ACCGACTCAG CGCTGAGATC AATCGGCCCG ACTATCTCGA CTTTGCCGAG TCTGGGCAGG
            781 TCTACTTTGG GATCATTGCC CTGTGACTCG AGGATCCACT AGTTCTAGAG CGGCCGCCAC
            841 CGCGGTGGAG CTCCAGCTTT TGTTCCCTTT AGTGAGGGTT AATTGCGCGC TTGGCGTAAT
            901 CATGGTCATA GCTGTTTCCT GTGTGAAATT GTTTATCCGC TCACAAATTC CACACAACAT
            961 ACGAGCCCGG GAAGCATAAA GTGTAAAGCC TGGGGTGCCC TAAATGAGTG AGCTACTCAC
           1021 ATTTAACTGC CGATGCGCTC ACTGGCCCGC TTTCCAGTCA GGCAACCTGA TCGTTGCCAG
          1081 CTGTGGCCAA TA
          //

          Please visit Sequencing and PCR primers for primer information.

          References

          Original, user report and related articles

          user_report Yokoyama, C., Interleukin-8 enhances the effect of colchicine on cell death. Biochem. Biophys. Res. Commun. 485 (1): 89-94 (2017). PMID 28189686.

          2023.05.02

          GNP_filter3_RDBDEP_html_230501.pl