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AxCA HSVtK

Recombinant adenovirus expressing Herpes simplex virus tk gene

Catalog number RDB01429
Resource name AxCA HSVtK
Alternative name Adex1CA HSVtK
Clone info. Recombinant adenovirus expressing herpes simplex virus tk gene regulated by CAG promoter.
Vector backbone Adex1 (Adenovirus)
Size of vector backbone -
Growth remarks 5 percent FCS is fine.
Gene/insert name HSV thymidine kinase cDNA
Depositor|Developer Hamada, Hirofumi |
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External Database
HSV thymidine kinase

          Reference sequence
            
           
          Remarks, protocol and/or map (gif) RDB01429.gif

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions for distribution The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
          Additional terms and conditions:
          Regarding resources containing CAG promoter: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the CAG promoter (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991) and an acknowledgment to Dr. Jun-ich Miyazaki of the Osaka University are requested.
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          MTA, for use for not-for-profit academic purpose [Word]
          Please visit Information of Request for Distribution.[link] 
          Remarks Please ensure RCAs are not in stocks prior to use of recombinant adenoviruses. (https://brc.riken.jp/dna/GRP0046e)
          提供案内 (日本国内) [open/close]

          提供条件 非営利機関の非営利学術研究へ提供する。利用者が本件リソースを利用した研究成果に基づき特許申請、商業活動等を行う場合は、利用者は事前に寄託者に連絡する。利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する。利用者は、研究成果の公表にあたって謝辞の表明を必要とする。
          付加的提供条件:
          CAGプロモータを含むリソースについて: 利用者は、研究成果の公表にあたってCAGプロモータの文献 (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991)を引用し、大阪大学 宮崎純一博士への謝辞の表明を必要とする。
          提供依頼
          提供依頼書 [Word]
          提供同意書 (MTA, 非営利学術目的用)[Word]
          遺伝子組換え生物の受入れ確認書 [Word]
          手続きの概要は、「提供申込みについて[link]」をご覧ください。
          備考 【追加提供依頼書】受入れ確認書(書式J)
          実験に使用する前に、タイターを上げた試料中にRCAが混入していないことを確認してください。(https://brc.riken.jp/dna/GRP0046j)
          組換え体提供にかかる書式が必要です。

          Catalog # Resource name Availability Shipping form Fee (non-profit org.)
          RDB01429 AxCA HSVtK Under QC test. Please contact us. Virus, lysate of infected cell culture

          check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

          How to cite this biological resource

          Materials & Methods section:

          The AxCA HSVtK was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB01429).

          Reference section:

          Shimizu, T., Shimada, H., Ochiai, T., Hamada, H., Enhanced growth suppression in esophageal carcinoma cells using adenovirus-mediated fusion gene transfer (uracil phosphoribosyl transferase and herpes simplex virus thymidine kinase). Cancer Gene Ther. 8 (7): 512-521 (2009). PMID 11498773.

          Further references such as user reports and related articles (go to bottom)


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          Featured content

          Featured content Suicide gene therapy (English text)

          QC test results


          References

          Original, user report and related articles

          original Shimizu, T., Enhanced growth suppression in esophageal carcinoma cells using adenovirus-mediated fusion gene transfer (uracil phosphoribosyl transferase and herpes simplex virus thymidine kinase). Cancer Gene Ther. 8 (7): 512-521 (2009). PMID 11498773.
          user_report Fukuda K., E1A, E1B double-restricted replicative adenovirus at low dose greatly augments tumor-specific suicide gene therapy for gallbladder cancer. Cancer Gene Ther., 16, 126-136 (2009). PMID 18818710. [link to RRC of NBRP]
          user_report Terazaki Y., An optimal therapeutic expression level is crucial for suicide gene therapy for hepatic metastatic cancer in mice. Hepatology, 37, 155-163 (2003). PMID 12500200. [link to RRC of NBRP]
          user_report Ugai H., Spontaneous mutations in the human gene for p53 in recombinant adenovirus during multiple passages in human embryonic kidney 293 cells. Biochem. Biophys. Res. Commun., 300, 448-456. (2003). PMID 12504105. [link to RRC of NBRP]

          2024.01.19

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